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Extended RNA Analysis (ERA) Platform

Available services (open to VUB and non-VUB)

RNA In Situ Hybridization

RNAscope/BaseScope represents a major advancement in RNA In Situ Hybridization with a specific probe design that allows to amplify target-specific signals in (archival) tissue or cultured cells from any species with single-molecule detection sensitivity while simultaneously suppressing background noise. While RNAscope targets longer (>300 bp) RNA, BaseScope targets short (50-300 bp) RNA including highly homologous sequences, exon junctions, splice variants and point mutations. Since probe design accounts for partial RNA degradation within the sample and the workflow does not require RNase-free conditions, processing of archival tissue such as clinical samples is possible. See page bottom for picture slideshow.

 

Available options:

Singleplex (1 target) with chromogenic or fluorescent detection

Multiplex (up to 4 targets) or HiPlex (up to 12 targets) with fluorescent detection

Can be combined with protein (antibody) staining

Full service from sample processing to image acquisition and quantitative analysis possible

 

For more information and a personalized free quote, contact: Yves.Heremans@vub.be

Download Flyer

Download ACD probe list

 

Automated RNA In Situ Hybridization & Immunostaining

Leica Biosystems BOND RX

For automated processing of up to 30 slides

Price: 13€/slide (single IHC)

Contact: Yves.Heremans@vub.be

 

Digital Image Acquisition & Quantitative Analysis

Invitrogen EVOS FL Auto Cell Imaging System

For acquisition of brightfield and 4-channel fluorescent images at 4-10-20-40x

Price: 5€/hr

Contact: Mathias.Van.Bulck@vub.be

 

IncuCyte ZOOM

For live cell imaging (phase contrast and 2-channel fluorescence at 4 or 10x), analysis and automated quantitation of cell behaviour over time (hours to weeks)

Price: 15€/plate/day

Contact: Philippe.Giron@vub.be

 

Indica Labs HALO Image Analysis Platform

For automated quantitative analysis of brightfield and fluorescent images

Price: 5€/hr

Contact: Mathias.Van.Bulck@vub.be

 

Testimonials

"Thanks to RNAscope performed at VUB, we were able to study in situ expression of the stem cell marker LGR5 in human colon, which would otherwise be impossible given the lack of reliable LGR5 antibodies."

A. Munoz, Instituto de Investigaciones Biomedicas "Alberto Sols", CSIC-UAM, Madrid

 

"We got a pretty positive experience with Yves' Team. The experiments were performed in a short time and we obtained very clear staining. RNAscope results were important to confirm our findings. Ultimately, Yves provided interesting advise and was very collaborative and available to answer our questions."

P. Jacquemin, de Duve Institute, Université catholique de Louvain, Brussels

 

“In collaboration with ACDBio and VUB, our laboratory designed a custom RNAscope probe against a target (Slc7a11/xCT) for which specific antibodies were lacking. The very first trial was succesfull and we moved on further to develop a fluorescent multiplex assay aiming at identifying the cellular expression.”

C. Nicaise, Namur Research Institute for Life Sciences, Université de Namur, Namur